A Study of Different Buffers to Maximize Viability of an Oral Shigella Vaccine
Live, oral vaccines against bacterial causes of diarrhea have to be formulated and administered in a way that protects them from the harsh acidic gastric environment. However, little work has been done to evaluate the volume of buffer needed to neutralize gastric acid while maintaining bacterial viability. This article, published in Vaccine, reports on study that aimed to determine the viability and stability of a Shigella sonnei oral vaccine candidate in a mixture of different volumes of five different buffer solutions added to hydrochloric acid to stimulate gastric acidity. The results showed that three of the tested buffers were better at neutralizing acid and maintaining viability of the vaccine, and that a much smaller volume was sufficient to counteract stomach acid while maintaining bacterial viability. ABSTRACT ONLY. (Learn how users in developing countries can gain free access to journal articles.)
Author: Chandrasekaran L, Lal M, Van De Verg LL, Venkatesan MM
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Citation: Chandrasekaran L, Lal M, Van De Verg LL, Venkatesan MM. A Study of Different Buffers to Maximize Viability of an Oral Shigella Vaccine. Vaccine. 2015;33(46):6156-6160.
Resource types: Peer-reviewed journal
Diseases/vaccines: Shigellosis and enterotoxigenic Escherichia coli (ETEC)